Released on: September 15, 2008, 7:24 am

Press Release Author: The Scott Partnership

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Press Release Summary: SANTA BARBARA, CALIFORNIA (September 15, 2008) - Wyatt
Technology, the world leader in absolute macromolecular characterization products,
announces the publication of a new, free-of-charge application note demonstrating
the capability of its DynaPro dynamic light scattering instrument to monitor the
dissociation of an oligomeric protein and unfolding of monomers simultaneously using
chaotropic agents. The analysis of proteins is particularly important for the study
of many diseases such as cancer, diabetes, cardiovascular disease and
neurodegenerative diseases such as Alzheimer's.

Press Release Body: NEW PROTEIN FOLDING APPLICATION NOTE USES WYATT TECHNOLOGY'S
DYNAMIC LIGHT SCATTERING TECHNIQUE

SANTA BARBARA, CALIFORNIA (September 15, 2008) - Wyatt Technology, the world leader
in absolute macromolecular characterization products, announces the publication of a
new, free-of-charge application note demonstrating the capability of its DynaPro
dynamic light scattering instrument to monitor the dissociation of an oligomeric
protein and unfolding of monomers simultaneously using chaotropic agents. The
analysis of proteins is particularly important for the study of many diseases such
as cancer, diabetes, cardiovascular disease and neurodegenerative diseases such as
Alzheimer's.

The importance of protein folding and unfolding has long been recognized and is an
active field of research. The main rules of protein folding/unfolding have been
established from in vitro experiments and the information obtained can be related to
the function and behavior of proteins in vivo. Unfolding of proteins in vitro can be
induced chemically using chaotropic agents like guanidine hydrochloride or urea.
Protein Y is a 16 kDa, almost fully helical human protein. At pH 5.0 it forms
oligomers of different sizes. Studying the unfolding of protein Y by circular
dichroism, the main transition in the secondary structure can be observed. The aim
of this specific study was to follow the unfolding process by another independent
method.

For this application, far UV CD spectra were taken at 25°C on an AVIV
spectrophotometer using a 0.01 cm cell. 5 spectra were recorded with a step size of
1.0 nm, a bandwidth of 1.5 nm and an averaging time of 5 seconds.
Samples were prepared 3 hours before measurement to allow time for the unfolding.
Protein concentration was 2 mg/ml. The inflexion point of the unfolding curve is at
2.6 M GnHCl by both methods. Dynamic light scattering was performed with a DynaPro
using a 12?l quartz cuvette. Samples were filtered with a 0.01?m Whatman Anodisc-13
filter.

Data were analyzed with the easy-to-use Wyatt Dynamics software and 25-30
acquisitions were averaged. The main transition in secondary structure can be
observed by estimating the Stokes radius of the protein under different
concentrations of the chaotropic agent (Fig. 1.). The mid-point of the transition
agrees exceptionally well with the CD data (2.6M GnHCl). However, applying DLS
measurements it is possible to observe additional molecular
events: the dissociation of oligomers in the 0-2 M GnHCl concentration range.

The results of the experiment clearly demonstrate that dynamic light scattering
measurements are capable of quickly and easily monitoring the dissociation of an
oligomeric protein and unfolding of monomers simultaneously using chaotropic agents.

For more information on Wyatt Technology's DynaPro or to obtain a copy of this
application note, please visit www.wyatt.com or e-mail info@wyatt.com

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About Wyatt
Based in Santa Barbara, California, Wyatt Technology is the world's leading provider
of instruments for absolute macromolecular characterization. With over 35 years'
experience developing multi-angle light scattering detectors, working with customers
in the biotechnology, chemical, petrochemical, pharmaceutical, academic and
government arenas, Wyatt prides itself on its entrepreneurial spirit, and the
uniqueness of its offerings. The Company's groundbreaking technology and
uncompromising levels of customer care make Wyatt the global hallmark in its field.
For more information, please visit www.wyatt.com

For further press information please contact: Laura Browne, The Scott Partnership, 1
Whiteside, Station Road, Holmes Chapel, Cheshire. CW4 8AA
United Kingdom Tel: + 44 1477 539539 Fax: +44 1477 539540 mail to:
wyatt@scottpr.com

Web Site: http://

Contact Details: Laura Browne, The Scott Partnership, 1 Whiteside, Station Road,
Holmes Chapel, Cheshire. CW4 8AA
United Kingdom Tel: + 44 1477 539539 Fax: +44 1477 539540 mail to:
wyatt@scottpr.com